Symposium Schedule
Nottingham Park Plaza Hotel
All talks include 5 minutes time for questions
| Wednesday, September 15 | |
|---|---|
| 09:00-10:00 | Registration |
| 10:00-10:15 | Opening |
| Session 1: Technical and methodological innovations in AUC | |
| Chair: Arthur Rowe | |
| 10:15-11:00 | Walter Stafford (Biochemical Society Lecture): Analysis of Interacting Systems -Time Derivative Sedimentation Analysis: DCDT, SEDANAL and the future. |
| 11:00-11:20 | Emre Brookes, Borries Demeler, Feng Ding, Nikolay V. Dokholyan and Mattia Rocco: Dealing with flexibility in biomacromolecular hydrodynamics: new interfaces within the UltraScan SOlution MOdeler (US-SOMO) suite for discrete molecular dynamics (DMD) and Brownian dynamics (BD) modules |
| 11:20-11:40 | Lance M. Hellman, Chunxia Zhao, Sidney W. Whiteheart and Michael G. Fried: Tracer Sedimentation Using Histidine-Tag-Specific Optical Probes |
| 11:40-12:00 | Xin Wang, Chongxu Zhang, Yueh-Chin Chiang, Shaun Toomey, Matthew Power, Roy Richardson, Mitch Granoff, Jeremy Bucklad-Dobbins, Susan Chase, Darren Lee, Clyde Denis, Tom Laue: AU-FDS characterization of protein-RNA complexes following single-step affinity purification |
| 12:00-13:15 | Conference photograph + Lunch |
| 13:15-14:45 | Poster Session* |
| 14:45-15:40 | Selected poster presentations (5 minutes + 1 question) |
| 15:40-16:00 | Tea Break |
| 16:00-16:45 | Don Winzor (J.M. Creeth Memorial Lecture): A fifty year venture into sedimentation equilibrium and protein self-association |
| Session 2: Innovations in Data Analysis | |
| Chair: Jose Garcia de la Torre | |
| 16:45-17:05 | John Philo: Excluding species from sedimentation velocity analysis via limiting the range of sedimentation coefficients: PBM versus g(s*) approaches revisited |
| 17:05-17:25 | Borries Demeler, Emre Brookes, Dan Zollars, Bruce Dubbs, Gary Gorbet: What’s new in UltraScan-III? |
| 17:25-17:40 | Emre Brookes, Borries Demeler: UltraScan: Improved 2DSA Resolution with Modified Parameter Space Grids. |
| 17:40-18:00 | Stephen Harding, Peter Schuck, Ali Saber Abdelhameed, Gordon Morris: Extended Fujita approach for molecular weight distribution analysis of polymers from sedimentation velocity |
| 18:00-18:15 | Antje Voelkel and Helmut Cölfen: Simultaneous determination of number and weight average molar masses of macromolecules by Analytical Ultracentrifugation |
| 18:30 | Bus leaves Plaza hotel for Sutton Bonington |
| 19:00 | Conference Dinner sponsored by Beckman-Coulter. Poster Prize award. |
| 22:00 | Bus returns to Plaza hotel |
| Thursday, September 16 | |
| Session 3: Hydrodynamics and modelling | |
| Chair: John Philo | |
| 09:00-09:20 | Alvaro Ortega, Jose G. Hernandez Cifre, Diego Amoros, Ricardo Rodriguez Schmidt and Jose Garcia de la Torre: Prediction of hydrodynamic properties of rigid and flexible particles: New advances and applications |
| 09:20-09:40 | Peter Zipper and Helmut Durchschlag: A Program Suite of Complementary Tools for Hydrodynamic and Scattering Modelling |
| 09:40-10:00 | Camillo Rosano and Mattia Rocco: Integrin αIIβ3 shape in solution: a multi-resolution study employing data from x-ray crystallography, NMR, EM, SANS, and AUC |
| 10:00-10:15 | Yuki Abe, Daniel G Bracewell, Jayesh Gor, Stephen J Perkins and Paul A Dalby: Masking of the Fc region in human IgG4 by constrained X-ray scattering modelling: implications for antibody function and therapy |
| 10:15-10:30 | Richard Berry, Simon Foulcer, Clair Baldock, Anthony Day and Thomas Jowitt: Using novel crystallohydrodynamic approaches to help define macro-molecular assembly |
| 10:30-11:00 | Coffee |
| Session 4: General polymers and colloids | |
| Chair: Tom Laue | |
| 11:00-11:20 | G. M. Pavlov, A. Wild, B. Happ, A. Winter and U. S. Schubert: New supramolecular structures studied through hydrodynamic investigations |
| 11:20-11:35 | Elena Tarabukina, Alina Amirova, Elena Belyaeva, Natalia Sheremeteva, Aziz Muzafarov and Alexander Filippov: Conformational properties of hyperbranched polycarbosilanes in solutions |
| 11:35-11:50 | C. Wandrey, H. Ahmadloo, F. Garcia, P. Ferreira and G. Rasteiro: Hydrodynamic analysis of permanently charged synthetic polyelectrolytes |
| 11:50-12:50 | Lunch |
| Session 5: Biological systems | |
| Chair: Borries Demeler | |
| 13:00-13:20 | Azubuike I Okemefuna, Penelope Adamson, Tania Sadlon, Jayesh Gor, David L. Gordon and Stephen J Perkins: A highly-looped central domain arrangement for complement Factor H provides insight on its regulatory mechanism |
| 13:20-13:40 | Oleksandr Brener, Luitgard Nagel-Steger and Dieter Willbold: Oligomeric species generated by amyloid beta peptide under low salt conditions |
| 13:40-13:55 | Grzegorz Piszczek, Gian Marco de Donatis, Michael R Maurizi: Global conformational changes of the ClpA chaperone hexamer studied by AUC |
| 13:55-14:10 | Trushar R. Patel, Gordon A. Morris, Danicla Zwolanek, Douglas R. Keene, Jianhua Li, Stephen E. Harding, Manuel Koch and Joerg Stetefeld: Solution conformation of laminin γ-1 short arm |
| 14:10-14:25 | Susumu Uchiyama, Masanori Noda, Adam McKay, Carol Robinson, Sachihiro Matsunaga and Kiichi Fukui: Assembly states of human nucleosome assembly protein 1 |
| 14:25-14:45 | Tea |
| Session 6: Interacting systems and assemblies | |
| Chair: Christine Wandrey | |
| 14:45-15:05 | Igor Perevyazko, Joseph T. Delaney Jr., Stephanie Schubert, Georges M. Pavlov and Ulrich S. Schubert: High-throughput nanoprecipitation: a new approach to developing and understanding self-assembling nanoparticles |
| 15:05-15:25 | Atul Saluja, R Matthew Fesinmeyer, Sabine Hogan, David N. Brems and Yatin Gokarn: The second virial coefficient from diffusional (kd) and sedimentation (ks) interaction parameters: Utility of kd as a high-throughput predictor of protein aggregation |
| 15:25-15:40 | Ruodan Nan, Georgia Ward, Louise Gavigan, Ami Miller, Jayesh Gor, Adam R McKay, Imre Lengyel and Stephen J Perkins: Comparison of the self-association and folded-back solution structure of the wild-type Tyr402 and the disease-related His402 allotypes of complement Factor H |
| 15:40-15:55 | Keying Li, Jayesh Gor and Stephen J Perkins: Self-association and domain rearrangements between complement C3 and C3u provide insight into the activation mechanism of C3 |
| 15:55 | Concluding remarks |
| 16:00 | End of Symposium |
*POSTERS
- **Roman S.G., Chebotareva N.A., Eronina T.B., Kleymenov S.Yu., Makeeva V.F., Kurganov B.I.: Effect of 2-hydroxypropyl-β-cyclodextrin on thermal aggregation of glycogen phosphorylase b
- John Philo: A new multi-section approach to scan differencing of SV data can be helpful for both time-derivative analysis and whole boundary modeling
- **Alvaro Ortega, Ramon Pamies, Kaizheng Zhu, Anna-Lena Kjoniksen, Bo Nystrom, Jose Garcia de la Torre: Analytical Ultracentrifugation of PNIPAAM-based diblock copolymers
- **Andre Ziegler, Joachim Seelig: Effects of glycosaminoglycan binding and clustering on biological uptake of cell-penetrating peptides
- **A. Mikhailova, A. Breul, C. Weber, M. Hager, R. Hoogenboom, G. M. Pavlov, U. S. Schubert: Hydrodynamic behavior of relatively short comb-like polymers
- **O.V. Okatova, N.N.Ulyanova, A.V.Mikhailova, I.I.Gavrilova, E.F.Panarin, G.M. Pavlov: Hydrodynamic properties and polyelectrolyte behavior of poly-N-methyl-N-vinylacetamide and vinyl amine hydrochloride copolymers in aqueous solutions
- **O. V. Okatova, L. N. Andreeva, I. A. Strelina, I. I. Gavrilova, E. F. Panarin, G. M. Pavlov: Molecular Characteristics of Polysaccharide Zosterin in Aqueous Solutions Obtained by Hydrodynamic and Optical Methods
- Helmut Durchschlag and Peter Zipper: Prediction of Individual Water Molecules and Water Channels by Modelling Approaches Based on Anhydrous 3D Models, Surface Calculations and Hydration Algorithms
- **Anita Niedziela-Majka, Ali R. Khaki, Cassandra Field, Shuja Malik, Stephanie Leavitt, Ruth Wang, Magdeleine Hung, Katherine M. Brendza, Christopher J. Fischer, Roman Sakowicz: Single ATPase site of Hepatitis C virus Helicase Controls Binding to Nucleic Acid and Directional Translocation along the Lattice.
- **Marcel Jurk, Matthias Dorn, Alexey Kikhney, Dmitri Svergun, Wolfgang Gaertner, Peter Schmieder: The Switch That Does Not Flip - Conformational Analysis of YtvA from Bacillus subtilis
- **David Besong, David Scott, Gary Adams, Arthur Rowe, Margaret Goodall, Roy Jefferis and Stephen Harding: The effect of glycosylation on solution conformation and storage stability of two IgG1 monoclonal antibodies
- Ali Saber Abdelhameed, Pierre Duvivier, Olivier Laloux, David Scott, Gary Adams, Arthur Rowe and Stephen Harding: Oligomeric state of the tetanus toxoid protein
- Gordon Morris, Samil Kok, Ali S. Abdelhameed, Shirley Ang and Stephen Harding: Global hydrodynamic analysis of the molecular flexibility of the dietary fibre polysaccharide glucomannan
- Gordon Morris, Samil Kok, Shirley Ang, Jana Kogulanathan, Peter Shewry, Arthur Tatham, Gary Adams, Arthur Rowe and Stephen Harding: Structure and heterogeneity of gliadin: a hydrodynamic evaluation
- **Antonio Molina-Garcia: Approaches to ice crystal dynamics control by molecular mobility
- **Arthur Rowe: Ultra-weak protein-protein interactions: a comparison of AUC and NMR approaches for their characterisation
** Selected for short presentation (5 minutes + 1 question)
Poster Prize judges:
Walter Stafford, Steve Perkins, Mattia Rocco, Georges Pavlov